2023 Fiscal Year Research-status Report
Elucidation of the effects of STB/HAP1 in modulating/protecting steroid hormone functions by acting on their receptors in the brain using genetically-modified mice
| Project/Area Number |
23K19456
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2023-08-31 – 2025-03-31
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| Keywords | HAP1 / steroid / Brain / rodent |
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| Outline of Annual Research Achievements |
Our previous in vitro findings suggest that STB/HAP1 could be related to the subcellular regulation of SHRs. In this research project to clarify the in vivo effects of STB/HAP1 in regulating or protecting the functions of sex steroids via their receptors; AR and ER, I examined the detailed in vivo immunohistochemical relationships of HAP1 with AR, and ER in the preoptic area and hypothalamic areas in adult mice. I found that HAP1 was highly coexpressed with both the sex steroid receptors in the different brain regions, which is suggestive of HAP1 modification of the steroidal functions in the brain.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
I have completed my first objective, clarifying the in vivo relationships of STB/HAP1 with different steroid receptors. I have presented my research findings at several academic conferences.
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| Strategy for Future Research Activity |
1. Assessments of effects of STB/HAP1 on serum hormone concentration.
2. Examination of effects of STB/HAP1 on the number of SHR-immunoreactive neurons.
3. Clarification of the effects of STB/HAP1 and steroid hormone-related functions.
4. Action if the research has yet to progress as planned initially.
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| Causes of Carryover |
In the present study, I am using knockout mice. The process of getting genetically modified mice was delayed due to the insufficient pregnancy rate of the mice.
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