2022 Fiscal Year Annual Research Report
Plasma-modified peptides/proteins for multi-target anticancer treatment
| Project/Area Number |
22H01212
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| Allocation Type | Single-year Grants |
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| Research Institution | Kyushu University |
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Principal Investigator |
Attri Pankaj 九州大学, プラズマナノ界面工学センター, 准教授 (40868361)
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| Co-Investigator(Kenkyū-buntansha) |
白谷 正治 九州大学, システム情報科学研究院, 教授 (90206293)
古閑 一憲 九州大学, システム情報科学研究院, 教授 (90315127)
田中 宏昌 名古屋大学, 低温プラズマ科学研究センター, 教授 (00508129)
竹内 希 東京工業大学, 工学院, 准教授 (80467018)
栗田 弘史 豊橋技術科学大学, 工学(系)研究科(研究院), 准教授 (70512177)
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| Project Period (FY) |
2022-04-01 – 2027-03-31
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| Keywords | COMSOL Multiphysics / CO2 and N2 plasma / Plasma Chemistry / Fluorescence / Plasma agriculture |
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| Outline of Annual Research Achievements |
We developed steamer discharge plasma working on N2, CO2, and Air feed gas. We also developed reaction chemistry for CO2, N2, and Air plasma to understand the production of short-lived species. Our 0D and 1D models are ready nowadays; we are focused on developing 2D models. 2D models in two different phases took a very long time to standardize due to complications in fluid flow in two distinct phases. Our 2D model is very close to the experimental conditions of the gas and liquid phase. OD and 1D model COMSOL model were helpful in predicting the very short reactive species those were are not possible to detect through the physical parameters. like We also measured the reactive species in the gas and liquid phase. Further, we measured the changes in the reactive species using a chemical analysis Kit and Fluorescence spectroscopy for the liquid phase analysis, and FT-IR used for the gas phase analysis. We analyzed our plasma system on the radish seeds to find out the change in germination index and growth. Also new analyzed the production of the NO3- species inside the seeds. We also checked the production of reactive production in the gas phase like CO, and O3 using FTIR. And detected the NH4+, NO3-, NO2- and H2O2 concentrations in the water using chemical analysis. Later, we also checked the production of OH radical in the water using the Fluorescence spectroscopy. The detection of OH, N2 second positive system, NO, CO, Alpha-H were detected in the gas phase using Optical emission spectroscopy. Our Simulation results are in the good agreement with the experimental results
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
According to the plan, we have developed CO2 and N2 streamer plasma to modify the peptides and proteins. Additionally, we also developed reaction chemistry to understand the production of short-lived species. Our 0D and 1D model is ready nowadays we are focused on developing 2D model. 2D models in two different phases took a very long time to standardize due to complications in fluid flow in two different phases.
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| Strategy for Future Research Activity |
We will use the cyclic dipeptides and treat them with plasma using Air, CO2, and N2 plasma. The modification in the peptides will be analysed by the variety of state-of-the-art analytical methods, such as circular dichroism, fluorescence spectroscopy, and mass spectrometry, again supported by computational methods, such as Molecular dynamics simulations, which will be used to understand the change in peptides. We will also use some synthesized organic drugs to interact with the protein and check the interaction using the MD simulation. We also plan to check the effect of catalase protein effect with and without inhibitors on the cancer cells. Later we evaluate the structural modification in the catalase enzyme using CD, fluorescence, and MS spectroscopy.
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