2023 Fiscal Year Annual Research Report
Thyroid hormones and Pigmentation pattern evolution in clownfish
| Project/Area Number |
22H02678
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| Allocation Type | Single-year Grants |
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
LAUDET Vincent 沖縄科学技術大学院大学, 海洋生態進化発生生物学ユニット, 教授 (20898423)
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| Co-Investigator(Kenkyū-buntansha) |
木下 政人 京都大学, 農学研究科, 准教授 (60263125)
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| Project Period (FY) |
2022-04-01 – 2026-03-31
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| Keywords | Pigmentation / Metamorphosis / Thyroid Hormones / Cora Reef Fishes / Ontogeny |
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| Outline of Annual Research Achievements |
Our purpose is to test the hypothesis that difference existing in pigmentation pattern ontogeny between two different clownfish species (Amphiprion ocellaris and A. percula) are linked to differences in thyroid hormone (TH) signaling. We have determined the ontogenetic transcriptome of these two species plus an outgroup and generated Crispr/Cas9 mutants of a gene controlling TH formation
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Because of the delay in setting-up our husbandry the main acheivements of our research has been delayed but we have now an excellent dataset of developmental transcriptomic to analyse and we have generated a Crispr/Cas9 line that show germline transmission. We are therefore very confident that important results will be generated very soon.
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| Strategy for Future Research Activity |
The work is now going into two main direction
1° We have transcriptomic data of A. ocellaris, A. percula and Premnas biaculeatus from hatching until the end of trunk bar formation (more than 100 days) post hatching. We have published the first part of this analysis for A. ocellaris in Roux et al., Cell Reports 2023. We are currently working on the comparative transcriptomic analysis of the three species.
2° In collaboration with our co-I Masato Kinoshita we have developped a Crispr/Cas9 line in A. ocellaris with a mutated version of the deiodinase 2 gene. These fishes should have less TH and exhibit a phenotype similar to A. percula. We have now founder fish that grow and will be crossed to generate homozygotes.
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