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2022 Fiscal Year Annual Research Report

Adaptation of marine zooplankton to climate change conditions: a multi-omic study of molecular mechanisms.

Research Project

Project/Area Number 22H03795
Allocation TypeSingle-year Grants
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

ラスカム ニコラス  沖縄科学技術大学院大学, ゲノム・遺伝子制御システム科学ユニット, 教授 (70724087)

Project Period (FY) 2022-04-01 – 2025-03-31
Keywordsbioinformatics / genomics / climate change / marine biology / plankton
Outline of Annual Research Achievements

- We showed using molecular, morphological and reproductive analysis that the Okinawa Oikopleura dioica is probably a different species or sub-species to Osaka specimen (Masunaga et al, 2022).
- We have recently completed the detailed genome comparisons between the Okinawa and Osaka Oikopleura dioica species (Plessy et al, Submitted).
- We have established comparative assays to measure the development rate of Oikopleura dioica in different saline concentrations.
- We have performed RNA-seq experiments of different time-points of exposure to different saline conditions.

Current Status of Research Progress
Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

- We are currently analysing the RNA-seq data.
- We are preparing to test further conditions (temperature, acidity).
- Maintaining constant temperatures over long periods is currently a technical challenge.

Strategy for Future Research Activity

- We are detecting clear phenotypic changes depending on the conditions.
- Our main focus is to understand the gene regulation underlying the adaptation of behaviours.
- Given the large number of cell types in an organism, we are currently testing single-cell RNA seq and cell line development.

  • Research Products

    (12 results)

All 2023 2022 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results)

  • [Int'l Joint Research] University of Barcelona(スペイン)

    • Country Name
      SPAIN
    • Counterpart Institution
      University of Barcelona
  • [Journal Article] The cosmopolitan appendicularian Oikopleura dioica reveals hidden genetic diversity around the globe2022

    • Author(s)
      Masunaga Aki、Mansfield Michael J.、Tan Yongkai、Liu Andrew W.、Bliznina Aleksandra、Barzaghi Paolo、Hodgetts Tamara L.、Ferrandez-Roldan Alfonso、Canestro Cristian、Onuma Takeshi A.、Plessy Charles、Luscombe Nicholas M.
    • Journal Title

      Marine Biology

      Volume: 169 Pages: -

    • DOI

      10.1007/s00227-022-04145-5

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] "Conservation of gene expression amidst genomic rearrangement in two species of Oikopleura dioica2023

    • Author(s)
      Michael Mansfield
    • Organizer
      Keystone Symposium: Epigenetics, Chromatin, Development, and Disease. Vancouver, Canada
    • Int'l Joint Research
  • [Presentation] Oikopleura dioica, the cosmopolitan appendicularian hides multiple cryptic species around the globe2022

    • Author(s)
      Aki Masunaga
    • Organizer
      11th International Tunicate Meeting (Kobe, Japan)
    • Int'l Joint Research
  • [Presentation] Drastic genome remodeling across Oikopleura dioica worldwide2022

    • Author(s)
      Charles Plessy
    • Organizer
      11th International Tunicate Meeting (Kobe, Japan)
    • Int'l Joint Research
  • [Presentation] Gene expression in the context of genomic rearrangement in Oikopleura dioica2022

    • Author(s)
      Michael Mansfield
    • Organizer
      11th International Tunicate Meeting (Kobe, Japan)
    • Int'l Joint Research
  • [Presentation] Massively scrambled genomes producing animals with conserved morphology2022

    • Author(s)
      Charles Plessy
    • Organizer
      45th Annual Meeting of the Molecular Biology Society of Japan (Chiba, Japan)
    • Int'l Joint Research
  • [Presentation] Related animals with small genomes, conserved morphology, but flexible gene order: Oikopleura dioica2022

    • Author(s)
      Charles Plessy
    • Organizer
      Oceans - and the impact of humans and climate change - OIST-Tohoku U. 2nd Joint Workshop on Marine Science
  • [Presentation] Uncovering speciation and population structures in the marine zooplankton, Oikopleura dioica2022

    • Author(s)
      Aki Masunaga
    • Organizer
      Oceans - and the impact of humans and climate change - OIST-Tohoku U. 2nd Joint Workshop on Marine Science
  • [Presentation] Drastic genome remodeling across Oikopleura dioica worldwide2022

    • Author(s)
      Charles Plessy
    • Organizer
      Konan University Department of Biology Seminar. Kobe, Japan
    • Invited
  • [Presentation] "Molecular clock estimation of divergence times in populations of "Oikopleura dioica"2022

    • Author(s)
      Michael Mansfield
    • Organizer
      Oceans - and the impact of humans and climate change. Onna-son, Japan
  • [Presentation] "Conservation of gene expression amidst genomic rearrangement in two species of Oikopleura dioica"2022

    • Author(s)
      Michael Mansfield
    • Organizer
      Konan University Department of Biology Seminar. Kobe, Japan
    • Invited

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Published: 2023-12-25  

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