腸内細菌叢を用いたコンディショニングの個別化に向けた基盤研究

2023 Fiscal Year Annual Research Report

• Project/Area Number: 23H03276
• Allocation Type: Single-year Grants
• Research Institution: Waseda University
• Principal Investigator: 谷澤 薫平 早稲田大学, スポーツ科学学術院, 准教授 (50771812)
• Co-Investigator(Kenkyū-buntansha): 鈴木 克彦 早稲田大学, スポーツ科学学術院, 教授 (80344597)
• Project Period (FY): 2023-04-01 – 2027-03-31
• Keywords: 腸内細菌叢 / コンディショニング / 炎症 / ストレス / 個人差 / 個別化

Outline of Annual Research Achievements

本研究課題では、腸内細菌叢が運動に対する炎症・ストレス応答やコンディション変化の個人差を規定するという仮説のもと、腸内細菌叢を用いたコンディショニングの個別化に向けた研究を行う。2023年度は、腸内細菌叢解析プロトコルを確立した上で、一過性運動に対する炎症・ストレス応答の個人差に関連する腸内細菌叢の特徴を明らかにするための研究に着手することを目標として研究を行った。

先行研究で報告された腸内細菌叢解析プロトコルを精査し、以下の通り糞便採取から群集構造解析までのプロトコルを決定した。1) 糞便採取：グアニジンチオシアン酸塩溶液入り採便容器を用いて糞便を採取し、DNA抽出まで常温で保存する。2) DNA抽出：糞便採取後1週間以内に、ビーズ破砕とスピンカラムを用いてDNA抽出を行う。3) ライブラリー作成：16S rRNA遺伝子のV4領域をアダプター付きプライマーを用いてPCR増幅後、磁性ビーズによりPCR産物を精製する。インデックス付きプライマーを用いたPCRにより、精製されたPCR産物にインデックスを付加し、再度磁性ビーズにより精製する。4)DNAシーケンス：次世代シーケンサーiSeq100を用いてDNAシーケンスを行う（24~48サンプルをマルチプレックスする）。5) リードのクオリティーコントロールとトリミング：FastQCとBBDukを用いて、リードのクオリティーコントロールとトリミングをそれぞれ行う。6) 群集構造解析：菌叢解析パイプラインQIIME2を用いて行う。
実際に上記のプロトコルを用いて解析を行ったところ、良好な結果が得られたため、今後はこのプロトコルを用いて腸内細菌叢の解析を行うことにした。

また、一過性運動に対する炎症・ストレス応答の個人差に関連する腸内細菌叢の特徴を明らかにするための研究について倫理申請を行った。

Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

本研究で用いる腸内細菌叢解析プロトコルを確立し、一過性運動に対する炎症・ストレス応答の個人差に関連する腸内細菌叢の特徴を明らかにするための研究について倫理申請を行ったが、実験開始には至らなかったため。

Strategy for Future Research Activity

2024年度は、一過性運動に対する炎症・ストレス応答の個人差に関連する腸内細菌叢の特徴を明らかにするため、年間を通じて実験を行う。また、高強度・高用量のトレーニングによるコンディション悪化の個人差に関連する腸内細菌叢の特徴を明らかにするために、本学の体育各部に研究協力を打診し、研究の準備を進める。

Research Products

• [Int'l Joint Research] Hungarian University of Sports Science(ハンガリー)
  • Country Name: HUNGARY
  • Counterpart Institution: Hungarian University of Sports Science
• [Journal Article] Dietary and other lifestyle factors and their influence on non-communicable diseases in the Western Pacific region (2024)
  • Author(s): Sun X, Yon DK, Nguyen TT, Tanisawa K, Son K, Zhang L, Shu J, Peng W, Yang Y, Branca F, Wahlqvist ML, Lim H, Wang Y.
  • Journal Title: The Lancet Regional Health - Western Pacific Volume: 43 Pages: 100842
  • DOI: 10.1016/j.lanwpc.2023.100842
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Association of skeletal muscle function, quantity, and quality with gut microbiota in Japanese adults: A cross‐sectional study (2024)
  • Author(s): Iwasaka C, Nanri H, Nakagata T, Ohno H, Tanisawa K, Konishi K, Murakami H, Hosomi K, Park J, Yamada Y, Ono R, Mizuguchi K, Kunisawa J, Miyachi M
  • Journal Title: Geriatrics & Gerontology International Volume: 24 Pages: 53～60
  • DOI: 10.1111/ggi.14751
  • Peer Reviewed
• [Journal Article] Associations between cardiorespiratory fitness and lifestyle‐related factors with <scp>DNA</scp> methylation‐based ageing clocks in older men: <scp>WASEDA</scp>'S Health Study (2024)
  • Author(s): Kawamura T, Radak Z, Tabata H, Akiyama H, Nakamura N, Kawakami R, Ito T, Usui C, Jokai M, Torma F, Kim HK, Miyachi M, Torii S, Suzuki K, Ishii K, Sakamoto S, Oka K, Higuchi M, Muraoka I, McGreevy KM, Horvath S, Tanisawa K
  • Journal Title: Aging Cell Volume: 23 Pages: e13960
  • DOI: 10.1111/acel.13960
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Association between physical activity and the prevalence of tumorigenic bacteria in the gut microbiota of Japanese adults: a cross-sectional study (2023)
  • Author(s): Iwasaka C, Ninomiya Y, Nakagata T, Nanri H, Watanabe D, Ohno H, Tanisawa K, Konishi K, Murakami H, Tsunematsu Y, Sato M, Watanabe K, Miyachi M
  • Journal Title: Scientific Reports Volume: 13 Pages: 2084
  • DOI: 10.1038/s41598-023-47442-9
  • Peer Reviewed / Open Access
• [Journal Article] Technology Innovation and Guardrails in Elite Sport: The Future is Now (2023)
  • Author(s): Guppy F, Muniz-Pardos B, Angeloudis K, Grivas GV, Pitsiladis A, Bundy R, Zelenkova I, Tanisawa K, Akiyama H, Keramitsoglou I, Miller M, Knopp M, Schweizer F, Luckfiel T, Ruiz D, Racinais S, Pitsiladis Y
  • Journal Title: Sports Medicine Volume: 53 Pages: 97～113
  • DOI: 10.1007/s40279-023-01913-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Association between circadian clock gene expressions and meal timing in young and older adults (2023)
  • Author(s): Takahashi M, Fukazawa M, Tahara Y, Kim HK, Tanisawa K, Ito T, Nakaoka T, Higuchi M, Shibata S
  • Journal Title: Chronobiology International Volume: 40 Pages: 1235～1243
  • DOI: 10.1080/07420528.2023.2256855
  • Peer Reviewed
• [Journal Article] The combined effect of rowing exercise and the intake of functional foods containing inulin on muscle mass and bone mineral density in older Japanese women (2023)
  • Author(s): Murata H, Zhang Q, Ito T, Kawakami R, Sakamoto S, Torii S, Shibata S, Tanisawa K, Higuchi M
  • Journal Title: Geriatrics &amp; Gerontology International Volume: 23 Pages: 779～787
  • DOI: 10.1111/ggi.14672
  • Peer Reviewed
• [Journal Article] Voluntary exercise does not increase gastrointestinal motility but increases spatial memory, intestinal eNOS, Akt levels, and Bifidobacteria abundance in the microbiome (2023)
  • Author(s): Bakonyi P, Kolonics A, Aczel D, Zhou L, Mozaffaritabar S, Molnar K, Laszlo L, Kutasi B, Tanisawa K, Park J, Gu Y, Pinho RA, Radak Z
  • Journal Title: Frontiers in Physiology Volume: 14 Pages: 1173636
  • DOI: 10.3389/fphys.2023.1173636
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ramen Consumption and Gut Microbiota Diversity in Japanese Women: Cross-Sectional Data from the NEXIS Cohort Study (2023)
  • Author(s): Park J, Bushita H, Nakano A, Hara A, Ueno HM, Ozato N, Hosomi K, Kawashima H, Chen YA, Mohsen A, Ohno H, Konishi K, Tanisawa K, Nanri H, Murakami H, Miyachi M, Kunisawa J, Mizuguchi K, Araki M
  • Journal Title: Microorganisms Volume: 11 Pages: 1892
  • DOI: 10.3390/microorganisms11081892
  • Peer Reviewed / Open Access
• [Journal Article] Acute social jetlag augments morning blood pressure surge: a randomized crossover trial (2023)
  • Author(s): Nakamura N, Akiyama H, Nishimura M, Zhu K, Suzuki K, Higuchi M, Tanisawa K
  • Journal Title: Hypertension Research Volume: 46 Pages: 2179～2191
  • DOI: 10.1038/s41440-023-01360-5
  • Peer Reviewed / Open Access
• [Journal Article] Body Cell Mass to Fat-Free Mass Ratio and Extra- to Intracellular Water Ratio Are Related to Maximal Oxygen Uptake (2023)
  • Author(s): Yamada Y, Yoshida T, Murakami H, Gando Y, Kawakami R, Ohno H, Tanisawa K, Konishi K, Tripette J, Kondo E, Nakagata T, Nanri H, Miyachi M
  • Journal Title: The Journals of Gerontology: Series A Volume: 78 Pages: 1778～1784
  • DOI: 10.1093/gerona/glad140
  • Peer Reviewed
• [Journal Article] DNA methylation clock DNAmFitAge shows regular exercise is associated with slower aging and systemic adaptation (2023)
  • Author(s): Jokai M, Torma F, McGreevy KM, Koltai E, Bori Z, Babszki G, Bakonyi P, Gombos Z, Gyorgy B, Aczel D, Toth L, Osvath P, Fridvalszky M, Teglas T, Posa A, Kujach S, Olek R, Kawamura T, Seki Y, Suzuki K, Tanisawa K, Goto S, Kerepesi C, Boldogh I, Ba X, Davies KJA, Horvath S, Radak Z
  • Journal Title: GeroScience Volume: 45 Pages: 2805～2817
  • DOI: 10.1007/s11357-023-00826-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An observational human study investigating the effect of anabolic androgenic steroid use on the transcriptome of skeletal muscle and whole blood using RNA-Seq (2023)
  • Author(s): Kolliari-Turner A, Lima G, Wang G, Malinsky FR, Karanikolou A, Eichhorn G, Tanisawa K, Ospina-Betancurt J, Hamilton B, Kumi PYO, Shurlock J, Skiadas V, Twycross-Lewis R, Kilduff L, Martin RP, Ash GI, Potter C, Guppy FM, Seto JT, Fossati C, Pigozzi F, Borrione P, Pitsiladis Y
  • Journal Title: BMC Medical Genomics Volume: 16 Pages: 94
  • DOI: 10.1186/s12920-023-01512-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] No strong association among epigenetic modifications by DNA methylation, telomere length, and physical fitness in biological aging (2023)
  • Author(s): Seki Y, Aczel D, Torma F, Jokai M, Boros A, Suzuki K, Higuchi M, Tanisawa K, Boldogh I, Horvath S, Radak Z
  • Journal Title: Biogerontology Volume: 24 Pages: 245～255
  • DOI: 10.1007/s10522-022-10011-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Coffee consumption and skeletal muscle mass: WASEDA’S Health Study (2023)
  • Author(s): Kawakami R, Tanisawa K, Ito T, Usui C, Ishii K, Muraoka I, Suzuki K, Sakamoto S, Higuchi M, Oka K
  • Journal Title: British Journal of Nutrition Volume: 130 Pages: 127～136
  • DOI: 10.1017/S0007114522003099
  • Peer Reviewed / Open Access
• [Journal Article] The MMAAS Project: An Observational Human Study Investigating the Effect of Anabolic Androgenic Steroid Use on Gene Expression and the Molecular Mechanism of Muscle Memory (2023)
  • Author(s): Lima G, Kolliari-Turner A, Wang G, Ho P, Meehan L, Roeszler K, Seto J, Malinsky FR, Karanikolou A, Eichhorn G, Tanisawa K, Ospina-Betancurt J, Hamilton B, Kumi PYO, Shurlock J, Skiadas V, Twycross-Lewis R, Kilduff L, Guppy FM, North K, Pitsiladis Y, Fossati C, Pigozzi F, Borrione P
  • Journal Title: Clinical Journal of Sport Medicine Volume: 33 Pages: e115～e122
  • DOI: 10.1097/JSM.0000000000001037
  • Peer Reviewed / Int'l Joint Research