2023 Fiscal Year Research-status Report
Molecular mechanisms of pathogenesis induced by endophytic bacteria of Brassica species in a nutrient-dependent manner
| Project/Area Number |
23KF0025
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| Research Institution | The University of Tokyo |
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Principal Investigator |
晝間 敬 東京大学, 大学院総合文化研究科, 准教授 (20714504)
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| Co-Investigator(Kenkyū-buntansha) |
UTAMI YUNIAR 東京大学, 大学院総合文化研究科, 外国人特別研究員
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| Project Period (FY) |
2023-04-25 – 2025-03-31
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| Keywords | 内生細菌 / 揮発性物質 / 栄養 / シロイヌナズナ / Pseudomonas |
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| Outline of Annual Research Achievements |
In this project, we are trying to understand the potential roles of plant-associated bacteria isolated from healthy plants with root colonization by root endophyte Colletotrichum tofieldiae.
We discovered that one of the strains, specifically a Pseudomonas strain (Psv), can be detrimental to plants through the production of unidentified volatile compounds.
If Psv is grown in rich media such as LB media, the nearby plants will be wilted despite any no physical interaction, suggesting the production of harmful volatiles by Psv. However, Psv can produce beneficial volatiles for plants when grown on low-nutrient media such as 1/3 LB. From our lab collection, We also identified a strain of Pseudomonas aeruginosa (Pso) isolated from Arabidopsis thaliana that produces both harmful and beneficial volatiles, exhibiting a phenotypic effect similar to that of Psv. Importantly, we found that in Pso, the harmful volatile production under rich media cultivation can weaken with quorum sensing lasR gene abrogation. We are currently performing the whole genome sequencing for Psv.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
In the reconstitution test, we found that Psv was contaminated. We then re-purified it into a single isolate. However, pure Psv isolate direct inoculation to plants causes wilt regardless of the media phosphate status, which is contradictory to our previous hypothesis. Still, the volatile production by Psv signifies its ability to exert pathogenicity depending on nutrient status. Thus, we need to change some of our experimental settings during this first year. Further, we found that Psv's colony characteristics lead to difficulty in extracting high-quality DNA samples for whole genome sequencing so it takes longer than expected before.
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| Strategy for Future Research Activity |
After the whole genome sequencing is finished, we would like to do the genome and transcriptome comparison with Pso under different nutrient conditions that switch their capabilities from harmful volatile producers into beneficial ones. We also seek to see plant responses to those different volatiles by RNA sequencing and find genes with potential function in volatile sensing and response.
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| Causes of Carryover |
RNAseq解析のためのサンプル条件を変更したため次年度に実験を行う計画へと変更した。
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