2023 Fiscal Year Research-status Report
Epigenetic regulation of cell cycle in plant meristem development
| Project/Area Number |
23KF0089
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
杉本 慶子 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (30455349)
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| Co-Investigator(Kenkyū-buntansha) |
HUNG FU-YU 国立研究開発法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員
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| Project Period (FY) |
2023-04-25 – 2025-03-31
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| Keywords | cell proliferation / pluripotent cells / callus / regeneration / histone acetylation / vernalization |
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| Outline of Annual Research Achievements |
In spring, plants produce a large number of new flower buds or leaf buds. This process is initiated by extensive cell proliferation and differentiation, which are primarily regulated during the preceding winter period. Extensive studies have uncovered the key molecular mechanisms of how long-term cold regulates flowering time, but how it modulates other processes is far less understood.In my study, I investigated how prolonged cold impacts plant regeneration and cell proliferation in epigenetic manner. We recently found that the formation of plant pluripotent cells called callus is enhanced in Arabidopsis seedlings that experienced vernalization. Interestingly, our transcriptome analysis suggests that the promotion of callus formation by vernalization is less-associated with auxin response, and instead is correlated with wounding response. Additionally, we found that several regeneration regulators are differentially expressed after vernalization and that their expression pattern is associated with changes in histone acetylation and H3K4 methylation, but not with H3K27 tri-methylation. Our data further show that the vernalization induced AP2-ERF transcription factors DREB1A/B/C (CBF1/2/3) can interact with histone acetyltransferase GCN5/HAG1, and play a critical role in promoting callus formation after vernalization by activating the core regeneration gene WOX5. Collectively, our results strongly suggest that plants exposed to prolonged cold increase the potential of pluripotent cell proliferation and promoting their re-differentiation.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Currently, I conducted a series of experiments to study the molecular mechanisms of how prolonged cold (vernalization) can increase the potential of pluripotent cell proliferation and promoting their re-differentiation. I have identified that transcription factors DREB1A/B/C (CBF1/2/3) and histone acetyltransferase GCN5/HAG1 complex is impotent to the activation of the core regeneration gene WOX5 to promoting callus formation and regeneration after vernalization. Now I’m also analyzing the global histone acetylation patterns regulated by CBFs-HAG1 by ChIP-seq, to further reveal their detailed molecular function.
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| Strategy for Future Research Activity |
In the recent future, I will study the genomic binding pattern of the histone acetyltransferase GCN5/HAG1 before or after vernalization treatment by the tatgeting-ChIPseq. These data will be compared with current histone modification ChIPseq and RNAseq datasets, to identify the functional correlation between the multi-omic sequencing data. With these analysis results, we can reveal the wider picture of how histone acetyltransferase regulating pluripotent cell proliferation and re-differentiation in plant system, and further understanding how long-term cold regulating the gene expression cascades. These results can help us to further understand how the “winter-effects” affecting plant development and reproduction. The finding from this study may provide some clues for us to deal with global climate change.
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| Causes of Carryover |
In the second financial year, I will perform several additional tatgeting-ChIPseq, histone modification ChIPseq and RNAseq assays. Meanwhile, I will also testing several developing protein-DNA interaction assays which designed for little amount of plant samples.
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