Structural basis for molecular mechanisms of membrane transporters

2016 Fiscal Year Final Research Report

• Project/Area Number: 24227004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: Nureki Osamu 東京大学, 大学院理学系研究科(理学部), 教授 (10272460)
• Co-Investigator(Kenkyū-buntansha): 伊藤 耕一 東京大学, 新領域創成科学研究科, 教授 (10262073) ; MATURANA ANDRES 名古屋大学, 生命農学研究科, 准教授 (10452004)
• Co-Investigator(Renkei-kenkyūsha): Maturana Andrés D. 名古屋大学, 生命農学研究科, 准教授 (10452004)
• Project Period (FY): 2012-05-31 – 2017-03-31
• Keywords: X線結晶構造解析 / チャネル / トランスポーター / 遺伝学 / 電気生理学

Outline of Final Research Achievements

Regarding ion channel, we solved high-resolution crystal structures of newly-identified Mg2+ channel MgtE, TRIC channel, P2X channel, and elucidated their substrate recognition mechanisms and gating mechanisms. For membrane transporters, we solved high-resolution crystal structures of Ca2+/H+ exchanger CAX, Fe2+ exporter FPN, sugar transporter SWEET, peptide transporter POT, amino acid exporter YddG, multi-drug exporter MATE, membrane protein integrator YidC to elucidate their substrate recognition mechanisms and transport mechanisms. We also solved high-resolution crystal structures of channel rhodopsin and light-driven Na+-pump KR2, which suggested how these rhodopsin channel/pump are gated upon light perception.

Free Research Field

構造生物学、生物物理学、生化学、分子生物学