Speaking Cell Approach by on-site/real-time cellular and molecular measurements

2016 Fiscal Year Final Research Report

• Project/Area Number: 24228004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Agricultural environmental engineering
• Research Institution: Ehime University
• Principal Investigator: NONAMI Hiroshi 愛媛大学, 農学研究科, 教授 (00211467)
• Co-Investigator(Kenkyū-buntansha): 平岡 賢三 山梨大学, クリーンエネルギー研究センター, 特任教授 (80107218)
• Co-Investigator(Renkei-kenkyūsha): MORITA Satoshi 独立行政法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, 上席研究員 (40391453) ; WADA Hiroshi 独立行政法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, 主任研究員 (40533146) ; NAKASHIMA Taiken 北海道大学, 大学院農学研究院, 助教 (70710945)
• Project Period (FY): 2012-05-31 – 2017-03-31
• Keywords: 細胞・組織 / 植物 / 質量分析 / 農業工学 / プレッシャープローブ / スピーキング・セル・アプローチ / 植物工場 / 細胞分子計測

Outline of Final Research Achievements

By using physiological molecular information, environmental conditions can be adjusted optimally to grow crops in plant growth factories. Such a control method uses physiological information to optimize energy efficiency and product quality control in plant growth factories, and is known as the “speaking cell approach” (SCA). In the present study, methods for on-site/real time cellular and molecular measurement techniques have been developed by using a cell pressure probe and probe electrospray ionization mass spectrometry for SCA. By mixing ionic liquid with the silicone oil in the pressure probe, pico pressure probe electrospray ionization mass spectrometry was developed to measure metabolite concentrations of single cells of plants. Hence, the water status and metabolite concentrations of single cells of plants can be measured simultaneously to evaluate the physiological conditions in real time to control environmental conditions in greenhouses.

Free Research Field

環境植物生理学