DNA barcoding applied to the analysis of water-quality indicator species and diversity of chironomid insects

2015 Fiscal Year Final Research Report

• Project/Area Number: 24241078
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Resource conservation science
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: Takamura Kenzi 国立研究開発法人国立環境研究所, 生物・生態系環境研究センター, フェロー (40163315)
• Co-Investigator(Kenkyū-buntansha): KONDO Natsuko 国立環境研究所, 生物・生態系環境研究センター, 主任研究員 (10414369) ; HIRABAYASHI Kimio 信州大学, 繊維学部, 教授 (20222250) ; KAWAI Koichiro 広島大学, 生物圏科学研究科, 教授 (30195028) ; UENO Ryuhei 国立環境研究所, 生物・生態系環境研究センター, 主任研究員 (60168648)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: DNAバーコード / 分子系統解析 / 種多様性 / 指標種 / 陸水 / COI

Outline of Final Research Achievements

DNA barcoding was applied to reveal the community composition of chironomids in various types of inland waters along with collecting DNA barcodes for molecular species identification. The DNA barcodes collected were made public in the chironomid DNA barcode databases both in Japanese and English. Several cryptic species were found by the molecular phylogenetic analysis during the course of DNA barcoding. On the community composition data mined by the DNA-based species identification, types of chironomid communities specific to a range of freshwater environments were classified and indicator species were found.

Free Research Field

生物多様性学