Peptide microarray for prognosis of cancer chemotherapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24245015
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyushu University
• Principal Investigator: KATAYAMA Yoshiki 九州大学, 工学(系)研究科(研究院), 教授 (70284528)
• Co-Investigator(Kenkyū-buntansha): MORI Takeshi 九州大学, 大学院工学研究院, 准教授 (70335785) ; ASAI Daisuke 聖マリアンナ医科大学, 微生物学教室, 助教 (10423485) ; OHUCHIDA Kenoki 九州大学, 大学院医学研究院, 講師 (20452708)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: プロテインキナーゼ / マイクロアレイ / ペプチド / 蛍光分析 / 薬物探索 / 診断 / キノーム

Outline of Final Research Achievements

Peptide microarray that can profile an intracellular kinome has been established. At first we successfully developed some new substrate peptides for various protein kinases which are important to evaluate effects of anti-cancer drugd. Then, by using these substrates, we prepared an peptide amicroarray and succeeded to get correct kinome profile against stimulation of drugs in living cells. Finally, kinomes of some different anti-cancer drugs, which have different specificity to EGFR and HER2, could be evaluated by the microarray.
For the analysis of tyrosine kinase activities, it was difficult to make specific substrates. Thus, we developed ligand peptides which are specfically bind to target tyrisine kinases. Using the ligand peptiddes, we successfully realize specific and sensitive monitoring of tyrosine kinase profiling.

Free Research Field

生体関連化学