Development of Organic Chemistry-based Method for Endogenous Protein Modification under Live Cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24245032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Kyoto University
• Principal Investigator: HAMACHI Itaru 京都大学, 工学(系)研究科(研究院), 教授 (90202259)
• Project Period (FY): 2012-05-31 – 2015-03-31
• Keywords: 生命化学 / 細胞系反応 / 蛋白質 / ラベル化

Outline of Final Research Achievements

The objective of this research project is development of novel chemistry-based methods for selective labeling of endogenous (natural) proteins not only in purified test tube, but also under crude live cell conditions. During the research, I succeeded in developing two of new ligand-directed chemistry, distinct from the previously established ligand-directed tosyl chemistry. The first is based on acyl-imidazole functional group (LDAI) which can be widely applied to membrane-bound proteins in live cells, and the second one exploits ortho-dibromophenyl-aryl ester as a cleavable functional group (termed as LDBB) that enables rapid labeling inside live cells.

Free Research Field

化学/生物学