Functional measurement and analysis of photosynthetic cells based on on-chip robotics

2014 Fiscal Year Final Research Report

• Project/Area Number: 24246045
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Nagoya University
• Principal Investigator: ARAI Fumihito 名古屋大学, 工学(系)研究科(研究院), 教授 (90221051)
• Co-Investigator(Kenkyū-buntansha): KAWAHARA Tomohiro 九州工業大学, 若手研究者フロンティア研究アカデミー, 准教授 (20575162)
• Co-Investigator(Renkei-kenkyūsha): MARUYAMA Hisataka 名古屋大学, 大学院工学研究科, 准教授 (60377843) ; UOZUMI Nobuyuki 東北大学, 大学院工学研究科, 教授 (40223515)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: マイクロ・ナノデバイス / 超精密計測 / バイオ関連機器 / 光ピンセット / ナノバイオ

Outline of Final Research Achievements

The purpose of this research is measurement of the multi-parameters of a single cyanobacteria, whose size is approximately 2 um in diameter, with high-throughput under local microfluidic environment control. Here, we constructed the systems for mechanical characterization and measurement of oxygen consumption of the cell. As for the cellular mechanical characterization, we constructed the system which enables us to transport the target cell using optical tweezers, and to measure its mechanical properties using the microfluidic chip which has on-chip robots. Using the constructed system, we investigated the effect of osmotic pressure stimulation to cyanobacteria and its mechanical properties. As for the measurement of oxygen consumption, florescent dye is utilized as sensors whose florescent intensity changes with respect to oxygen concentration. We fabricated a stripe oxygen sensor into the microchamber, and successfully measured the oxygen concentration distribution of confined space.

Free Research Field

ナノマイクロメカトロニクス，ロボティクス