Development of biological immmunoassay system using magnetic markers

2015 Fiscal Year Final Research Report

• Project/Area Number: 24246072
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Measurement engineering
• Research Institution: Kyushu University
• Principal Investigator: ENPUKU KEIJI 九州大学, 超伝導システム科学研究センター, 教授 (20150493)
• Co-Investigator(Kenkyū-buntansha): YOSHISA TAKASHI 九州大学, システム情報科学研究院, 准教授 (30380588) ; KUMA HIROYUKI 長崎国際大学, 薬学部, 准教授 (40435136) ; INOUE MASAYOSHI 九州大学, システム情報科学研究院, 准教授 (80346824) ; SASAYAMA TERUYOSHI 九州大学, システム情報科学研究院, 助教 (60636249)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 免疫検査 / 磁気マーカー / SQUIDセンサ / 磁気ナノ粒子 / ブラウン緩和 / 磁気粒子イメージング

Outline of Final Research Achievements

We have developed a biological immunoassay technique utilizing magnetic markers and highly sensitive magnetic sensors. First, we quantitatively characterized the properties of magnetic markers. These results can be used to find markers suitable for bio-application. Next, we developed a liquid-phase detection method of biological targets, where bound and free markers are magnetically distinguished by using Brownian relaxation. Therefore, we can omit a time consuming washing process called bound/free separation. We demonstrated highly sensitive detection of biological targets using this technique. Finally, we developed a magnetic-particles-imaging system for the application to in-vivo diagnosis. Magnetic particles, that were placed 50 mm below the sensors, were successfully detected with high sensitivity and spatial resolution. These results indicated the usefulness of the present systems.

Free Research Field

電気電子工学・計測工学