Structural basis for ubiquitin chain-mediated DNA damage responses

2015 Fiscal Year Final Research Report

• Project/Area Number: 24247014
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Fukai Shuya 東京大学, 放射光連携研究機構, 准教授 (10361792)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 分子認識 / DNA修復 / タンパク質複合体 / ユビキチン / Ｘ線結晶構造解析 / 構造生物学

Outline of Final Research Achievements

Ubiquitin serves as a signaling molecule for regulation of protein degradation and other important cellular functions. In many cases, two or more ubiquitin molecules are tandemly linked through covalent bonding to function as polyubiquitin chains. In this research project, we determined three-dimensional structures of protein complexes that play important roles in synthesis, degradation and recognition of K63-linked polyubiquitin chains (K63 chains) for DNA double strand break (DSB) responses to elucidate mechanisms for the K63 chain-mediated regulation of the DSB responses at atomic resolution. Furthermore, we revealed the structure-function relationships through structure-guided mutational analyses at the molecular and cellular levels.

Free Research Field

構造生物学