2015 Fiscal Year Final Research Report
Analysis of potential stem cells that change their fate from differentiation to self-renewal and support the regeneration of spermatogenesis
Project/Area Number |
24247041
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Developmental biology
|
Research Institution | National Institute for Basic Biology |
Principal Investigator |
YOSHIDA Shosei 基礎生物学研究所, 生殖細胞研究部門, 教授 (60294138)
|
Project Period (FY) |
2012-05-31 – 2016-03-31
|
Keywords | 生殖細胞 / 分化 / 脱分化 / 幹細胞 |
Outline of Final Research Achievements |
Using mouse spermatogenesis, his study investigated the “potential stem cells” that largely differentiate without self-renewal under a normal condition but revert back to self-renewal during regeneration after tissue damage. The discoveries made in this study include: 1) In response to retinoic acid (RA), a differentiation-inducing signal, while GFRα1+ spermatogonia (cells supporting the steady state) remain undifferentiated, while Ngn3+ cells (those acting as potential stem cells) undergo irreversible differentiation and lose the stem cell potential. 2) This differential RA responsiveness is caused by the differential expression of RARg (a RA receptor gene) only in the Ngn3+ cells. These findings suggest a novel regulatory mechanism for the robust stem cell systems.
|
Free Research Field |
発生生物学、生殖細胞生物学、幹細胞生物学
|