Quick isolation of rice QTL by whole genome sequencing

2014 Fiscal Year Final Research Report

• Project/Area Number: 24248004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: TERAUCHI Ryohei 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 部長 (50236981)
• Co-Investigator(Kenkyū-buntansha): ISHIURA Masahiro 名古屋大学, 遺伝子実験施設, 教授 (20132730) ; ONAI Kiyoshi 名古屋大学, 遺伝子実験施設, 教授 (00402454)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 全ゲノム解析 / QTL / イネ / 生物発光リアルタイム測定 / シロイヌナズナ

Outline of Final Research Achievements

Aiming at efficient breeding of rice cultivars, we applied QTL-seq method (Takagi et al. Plant J. 74:174) to rice to identify quantitative trait loci (QTL) controlling important agronomic traits, resulting in successful identification of QTL for cold tolerance, heading date, and partial resistance to blast disease. We further attempted to combine QTL-seq with a high-throughput luciferase bioluminescence measurement system to identify QTL governing WRKY19 gene promoter activity in Arabidopsis, with a promising preliminary result.

Free Research Field

植物遺伝学