Regulatory mechanisms of plant surface signal reception by plant pathogenic fungi during host infection

2014 Fiscal Year Final Research Report

• Project/Area Number: 24248009
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kyoto Prefectural University
• Principal Investigator: KUBO Yasuyuki 京都府立大学, 生命環境科学研究科(系), 教授 (80183797)
• Co-Investigator(Kenkyū-buntansha): TAKANO Yoshitaka 京都大学大学院, 農学研究科, 准教授 (80293918)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 炭疽病菌 / 侵入様式 / 付着器 / シグナル伝達 / RNA結合タンパク質 / RNAサイレンシング / 分子パターン / 植物免疫

Outline of Final Research Achievements

It has been generally accepted that Colletotrichum species form appressoria as an infection structure through perception of plant surface signals such as hydrophobicity and hardness. In this study, we revealed that octadecanal, a cutin monomer derived from plant leaf surface triggers C. orbiculare to develop infection structure through MOR signal transduction pathway composed of CoPAG1 and CoCBK1. In addition, we showed that PacC, an environmental signal response factor, is involved in regulation of secretion proteins gene expression that are essential for pathogens in HTE mode of infection as well as appressorium-mediated infection. Furthermore, we evaluated the involvement of RNA silencing in pathogenesis and showed that Dicer-like gene DCL2 is essential for pathogenesis and identified DCL-2 dependent small RNAs.

Free Research Field

植物病理学