Construction of the database of microbial products and its application study

2014 Fiscal Year Final Research Report

• Project/Area Number: 24248022
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OSADA Hiroyuki 独立行政法人理化学研究所, 環境資源科学研究センター, 副センター長 (80160836)
• Co-Investigator(Renkei-kenkyūsha): MUROI Makoto 独立行政法人理化学研究所, 環境資源科学研究センター, 上級研究員 (30261168) ; NOGAWA Toshihiko 独立行政法人理化学研究所, 環境資源科学研究センター, 研究員 (40462717)
• Project Period (FY): 2012-05-31 – 2015-03-31
• Keywords: 生物活性物質 / 微生物代謝産物 / プロテオーム / フラクションライブラリー / データベース / MPPlot / ChemProteoBase / NPPlot

Outline of Final Research Achievements

In order to overcome disadvantage of natural products as a source for drug screening, we established a technical platform based on chemical biology. In this study, we constructed a fraction library from microbial and plant sources based on basic chromatographic techniques, such as MPLC and HPLC, combined with a database named Natural Products Plot (NPPlot). NPPlot is a distribution map of metabolites, which are plotted as a dot in a three-dimensional area with X-, Y-, and Z-axis for retention time, m/z value, and UV absorption, respectively. Using this database, characteristic metabolites are easily found for each of strain. Furthermore, we developed a drug target identification system based on proteomic analysis by 2-D gel electrophoresis, ChemProteoBase. Isolation of novel natural compounds and prediction of their targets would be accelerated by using our systems, NPPlot and ChemProteoBase. Our research is expected to contribute to drug discovery and progress of bioscience.

Free Research Field

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