Study on hepatic liver accumulation and SREBP-1 activation, and function of food factors

2014 Fiscal Year Final Research Report

• Project/Area Number: 24248023
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Ryuichiro 東京大学, 農学生命科学研究科, 教授 (50187259)
• Co-Investigator(Renkei-kenkyūsha): SHIMIZU Makoto 東京大学, 大学院農学生命科学研究科, 助教 (40409008)
• Project Period (FY): 2012-05-31 – 2015-03-31
• Keywords: SREBP-1 / ChREBP / 脂肪肝 / スプライシング / 果糖

Outline of Final Research Achievements

The evaluation system for measuring absolute value of ChREBPalpha/beta expression was established. ChREBPalpha/beta expression was observed in adipose tissues, liver, and small intestine, whereas only ChREBPalpha expression was detected in skeletal muscle. When ChREBPalpha/beta were overexpressed in cultured hepatocytes, lipogenic gene expression was significantly increased by ChREBPbeta. Mice overexpressing ChREBPbeta in the liver developed fatty liver. Four week feeding of a fructose-containing diet to mice resulted in a significant increase in hepatic ChREBPbeta expression and liver lipid accumulation. No alteration of hepatic SREBP-1c indicates that SREBP-1c was not involved in lipid accumulation induced by ChREBPbeta.

Free Research Field

食品生化学