Development of forest paleogenetic approaches using ancient pollen grains found in an ice core from mountain glacier

2015 Fiscal Year Final Research Report

• Project/Area Number: 24248025
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Forest science
• Research Institution: Tohoku University
• Principal Investigator: Suyama Yoshihisa 東北大学, (連合)農学研究科(研究院), 准教授 (60282315)
• Co-Investigator(Renkei-kenkyūsha): NAKAZAWA Fumio 大学共同利用機関法人情報・システム研究機構, 新領域融合研究センター, 融合プロジェクト特任研究員 (80432178) ; MAKINO Takashi 東北大学, 生命科学研究科, 准教授 (20443442)
• Research Collaborator: MATSUKI Yu 東北大学, 大学院農学研究科, 研究支援者
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 古代DNA / 遺伝的多様性 / 花粉分析 / 次世代シーケンサー / アイスコア / 集団遺伝

Outline of Final Research Achievements

We developed basic methods for paleogenetic approaches using conifer pollen grains found in a mountain glacier. First, we developed a protocol for single-pollen DNA analysis using whole-genome amplification (WGA) method. Second, we developed a novel approach that we termed “multiplexed ISSR genotyping by sequencing” (MIG-seq), which is a PCR-based procedure for constructing highly reduced representation libraries involving de novo SNP discovery, and their genotyping using next generation sequencing. Then we applied these methods to pollen samples found in a glacier.

Free Research Field

森林分子生態学