2014 Fiscal Year Final Research Report
Elucidation of RNA interference in fish and shellfish and its application for aquaculture and pathogen defense
Project/Area Number |
24248034
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General fisheries
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Research Institution | The University of Tokyo |
Principal Investigator |
ASAKAWA Shuichi 東京大学, 農学生命科学研究科, 教授 (30231872)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | RNA干渉 / miRNA / piRNA / torafugu / medaka / pearl oyster / Heterobothrium okamotoi / トランスクリプトーム |
Outline of Final Research Achievements |
In this study, we comprehensively analyzed the small RNA involved in RNA interference in fish and shellfish to get basic knowledge about RNAi of the aquatic organisms and utilize the knowledge in the field of fisheries. By the transcriptome using next-generation sequencer, we revealed small RNA expression profile in each tissue of the torafugu (Takifugu rubripes), medaka (Oryzias latipes) and pearl oyster (Pinctada fucata). We found 1420 miRNA species in torafugu, and then found existence of large number of isomiRs for each of miRNA. We had an idea to take advantage of the RNAi in pathogen defense of fish and shellfish. For this, we analyzed the genomic sequences of pathogenic organisms.
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Free Research Field |
水圏生物工学
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