Cell-cell communications in developing skeletal muscle

2014 Fiscal Year Final Research Report

• Project/Area Number: 24248045
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Hokkaido University
• Principal Investigator: NISHIMURA Takanori 北海道大学, (連合)農学研究科(研究院), 教授 (10237729)
• Co-Investigator(Kenkyū-buntansha): TATSUMI Ryuichi 九州大学, 農学研究院, 准教授 (40250493) ; OJIMA Koichi 独立行政法人農業, 食品産業技術総合研究機構・畜産草地研究所, 畜産物研究領域 (60415544) ; HOSAKA Yoshinao 鳥取大学, 農学部共同獣医学科, 教授 (00337023)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 骨格筋 / 筋細胞 / 脂肪細胞 / 神経細胞 / 生理活性因子

Outline of Final Research Achievements

As skeletal muscle tissue consists of not only muscle cells but also other types of cells, including nerve cells and fat cells, the formation of skeletal muscle must be precisely regulated by these types of cells. Thus, we investigated the mechanisms of cell-cell communications in skeletal muscle. Myogenic cells secreted more than 400 proteins and approximately 8% of them were categorized as myokines. The levels of myokines that promote myotube formation peaked during early differentiation, whereas myokines contributing to repellent activity against nerve cells or suppression of adipogenesis decreased after differentiation. Myostatin secreted from myotubes suppressed the lipid accumulation in adipocytes. Some extracellular molecules modulated the action of myostatin to adipocytes. Satellite cells, resident myogenic stem cells, controlled neuritogenesis by up-regulating a secreted neural chemorepellent semaphorin 3A, which were induced by HGF that was secreted from macrophages.

Free Research Field

筋細胞生物学