2014 Fiscal Year Final Research Report
Identification of human bone marrow niche to support HSCs
| Project/Area Number |
24249056
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 骨髄ニッチ / TGF-beta / 休眠状態 |
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| Outline of Final Research Achievements |
This project describes identification of human bone marrow niche to support HSCs. We hypothesized that identification of BM niche leads us to manipulate the function of human HSPCs in vivo and in vitro. Given that TGF-beta induces human HSPC hibernation, we analyzed the area where TGF is activated. Our result clearly showed that GFAP+ cells activate TGF and induce hibernation of human HSPCs in mouse BM. These findings were confirmed by the fact that nerve denervation reduces the number of human HSPCs in human BM. Furthermore, We first report suggesting an association between neural and hematopoietic systems.In this paper, we first described an innovative strategy to generate large numbers of HSCs from pluripotent stem cells. More specifically, we established in vivo differentiation system generating fully functional HSCs from mouse induced pluripotent stem cells. Using this system, we also succeeded in generating human HSCs from human iPSCs.
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| Free Research Field |
再生医療
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