2015 Fiscal Year Final Research Report
HCN4-luciferase knock-in mouse is a new tool for pacemaker cell reprogramming
| Project/Area Number |
24300145
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Masayuki 久留米大学, 医学部, 助教 (20442535)
TAKEYA Mitsue 久留米大学, 医学部, 助教 (30289433)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMASHITA Jun 京都大学, iPS細胞研究所, 教授 (50335288)
KUWAHARA Koichiro 京都大学, 医学研究科, 講師 (30402887)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 洞房結節 / ペースメーカー細胞 / イオンチャネル |
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| Outline of Final Research Achievements |
As a next-generation therapy for bradyarrhythmia, development of biologically engineered pacemaker cells is anticipated. HCN4 channel is widely known as a specific molecular marker for the pacemaker cell of sinoatrial node. In order to visualize HCN4-expressing bio-pacemaker cell, we generated transgenic mouse, in which cDNA of firefly luciferase-GFP fusion protein was knocked in at HCN4 locus (HCN4+/Luc). 15 min after intraperitoneal injection of 50 mg/kg luciferin, we could successfully visualized HCN4-expresssing myocyte in SAN area. Weak but significant luminescence of cultured neonatal ventricular myocyte (NVM) could be also detected using LAS4000 system. However, lentiviral transfection of Tbx3 or Tbx18 into NVM failed to increase HCN4-derived luminescence.
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| Free Research Field |
生理学
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