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2014 Fiscal Year Final Research Report

Onset and persistent mechanism of retrovirus induced thrombocytopenia in Japanese macaques.

Research Project

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Project/Area Number 24300153
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionKyoto University

Principal Investigator

OKAMOTO Munehiro  京都大学, 霊長類研究所, 教授 (70177096)

Co-Investigator(Kenkyū-buntansha) AKARI Hirofumi  京都大学, 霊長類研究所, 教授 (20294671)
Co-Investigator(Renkei-kenkyūsha) ISA Tadashi  生理学研究所, 生命化学研究科, 教授 (20212805)
SUZUKI Juri  京都大学, 霊長類研究所, 准教授 (10175408)
MATSUOKA Masao  京都大学, ウイルス研究所, 教授 (10244138)
MIYABE Takako  京都大学, 霊長類研究所, 助教 (10437288)
SUZUKI Juri  京都大学, 霊長類研究所, 助教 (80418681)
YOSHIDA Tomoyuki  京都大学, 霊長類研究所, 助教 (00451948)
SATO Eiji  京都大学, 霊長類研究所, 助教 (90623918)
Project Period (FY) 2012-04-01 – 2015-03-31
Keywordsニホンザル / 血小板減少症 / SRV-4 / 発症機序 / 感染実験
Outline of Final Research Achievements

We isolated SRV-4 strain PRI-172 from a Japanese macaque showing severe thrombocytopenia. When inoculated into four Japanese macaques, the isolate induced severe thrombocytopenia in all within 37 days. We then constructed an infectious molecular clone of strain PRI-172, and inoculated the clone-derived virus into two Japanese macaques. These animals also developed severe thrombocytopenia in just 31 days after inoculation, and the virus was re-isolated from blood, bone marrow and stool. At necropsy, we observed bleeding from gingiva, and subcutaneous bleeding in all animals. SRV-4 infected various tissues especially in digestive organs including colon and stomach. We identified the SRV-4 receptor as ASCT2. ASCT2 mRNA was expressed in various tissues and the distribution of SRV-4 proviruses correlated well with the expression levels of ASCT2 mRNA. From these results, we conclude that the causative agent of hemorrhagic syndrome in Japanese macaques was SRV-4, and its receptor is ASCT2.

Free Research Field

分子感染症学

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Published: 2016-06-03  

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