Development of MEMS Devices with Microwell Slide for High Throughput Analysis of Cell Culture Experiment

2014 Fiscal Year Final Research Report

• Project/Area Number: 24300157
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Hokkaido University
• Principal Investigator: OHASHI Toshiro 北海道大学, 工学(系)研究科(研究院), 教授 (30270812)
• Co-Investigator(Kenkyū-buntansha): MAEDA Eijiro 北海道大学, 大学院工学研究院, 助教 (20581614)
• Research Collaborator: ANDERSSON-SVAHN Helene Royal Institute of Technology, Sweden, Prof. ; COOPER-WHITE Justin The University of Melbourne, Australia, Prof.
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細胞培養実験 / マイクロウェルスライド / マイクロフルイディクス / バイオチップ / バイオMEMS

Outline of Final Research Achievements

In this project, MEMS devices that allow to realize measurements and control of physical environment of cells were developed. The developed MEMS devices are for experiments of cell responses to mechanical environment, cell responses to chemical environment and cell migration. For the experiment of cell mechanical responses, it was confirmed to apply different level of fluid shear stress to endothelial cells cultured in a microwell to perform flow experiments. For the experiment of cell chemical responses, it was confirmed to apply different concentration of Saponin solution to endothelial cells to perform live/dead cell assay. For cell migration assay, mechanical characterization of fibroblast migration could be performed by using a micropillar array.

Free Research Field

バイオメカニクス