Development of 3D cultivation system that enables to control "epigenetic change" of the cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24300160
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyoto University
• Principal Investigator: ETO Koji 京都大学, iPS細胞研究所, 教授 (50286986)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: エピゲノム / ３D培養系 / ずり応用 / iPS細胞 / 造血幹細胞 / 巨核球 / 赤芽球 / 血小板

Outline of Final Research Achievements

Comparison between static and 3D conditions allowed us to conclude that physical irritant action including shear stress to contribute to differentiation to a cell growth as well as specific cell genealogy (lineage decision, for example, from a hematopoietic system cell [HSC] to megakaryocyte) and last maturity phase (i.e., platelet production from megakaryocyte). This implicated the importance of culture circumstances towards clinical application, in particular using from pluripotent stem cells. On the other hand, there is the problem of the wide “heterogeneity” of the cells during cell culture. We simultaneously clarified the importance of single cell analyzing technology useful for lineage commitment and controllable differentiation. Development of analysis technology by the single cell levels was associated with development of this research task.

Free Research Field

血小板、幹細胞生物学