Multi dimensions control of a nanofiber structure for regenerative therapy and its application to cornea regeneration

2014 Fiscal Year Final Research Report

• Project/Area Number: 24300176
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: National Institute for Materials Science
• Principal Investigator: KOBAYASHI Hisatoshi 独立行政法人物質・材料研究機構, 生体機能材料ユニット, グループリーダー (90354266)
• Co-Investigator(Renkei-kenkyūsha): HATTORI Shinya 物質・研究機構, 生体機能材料ユニット, ポスドク研究員 (30469762)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 角膜再生材料 / ナノファイバー高次構造化 / 生体タンパク質

Outline of Final Research Achievements

In this study, we studied basic technology for an extremely similar structure with corneal structure, by using collagen and silk materials which we have studied. Using electrospinning method, we developed epithelium basal membrane similar nanofiber scaffolding. And we tried to develop the transparency improved nanofiber. And we got fundamental data about high intensity Descemet's membrane structure regeneration.
Turning fiber size down to a level of nano, and making the comfortable space between the nanofibers(cell Niche), the light scattering had been minimized, then the transparency was improved. Higher order structures, surface characters, and hardness of the fibers, were strongly affect to the responses of both corneal epithlium and Keratocyte.

Free Research Field

生体材料学