Exploring food-derived factors that affect skeletal muscle metabolism for prevention of chronic diseases

2015 Fiscal Year Final Research Report

• Project/Area Number: 24300254
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Ochanomizu University
• Principal Investigator: Iida Kaoruko お茶の水女子大学, 基幹研究院, 准教授 (50375458)
• Co-Investigator(Kenkyū-buntansha): KONDO Kazuo 東洋大学, 食環境学部, 教授 (30153711) ; SUZUKI Emiko お茶の水女子大学, 基幹研究院, 教授 (80154524)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 骨格筋 / 食品因子 / 肥満 / ミトコンドリア / エネルギー代謝 / 慢性炎症

Outline of Final Research Achievements

This study aimed to identify food-derived factors that affect muscle metabolism in obesity. For this purpose, we used the C2C12 murine muscle cell line. Screening based on the promoter assay suggested that soy isoflavone daidzein was a potent candidate that regulated mitochondrial biogenesis. Treatment of C2C12 myotubes with daidzein resulted in significant increases in the expression of mitochondrial transcription factor A (Tfam) as well as cytochrome b and COX1 as its targets. We found that the nuclear respiratory factor (NRF) were necessary for the effect of daidzein on Tfam expression. In addition, daidzein regulated cytokine expression through the PPAR-α and -γ in animal adipose tissue and cultured adipocytes, thereby improving the adverse effects of adipose inflammation in obesity. We also examined the effect of ketogenic nutrient environment on muscle metabolism in mice and found that ketotic states promote transcriptional regulation of various metabolic genes in muscles.

Free Research Field

応用栄養学