Role of nucleolin in maintenance of genomic stability through chromatin remodeling

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: KOBAYASHI Junya 京都大学, 放射線生物研究センター, 准教授 (30301302)
• Co-Investigator(Kenkyū-buntansha): OKUI Michiyo 桐蔭横浜大学, 先端医用工学センター, 講師 (20327654) ; HAYASHI Ikue 広島大学, 医歯薬保健学研究院, 助教 (00346503)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ゲノム不安定性 / nucleolin / クロマチン / DNA損傷 / DNA修復

Outline of Final Research Achievements

We identified nucleolin (nucleolar protein) as one of H2AX-binding proteins. As nucleolin could remodel chromatin structure at transcription-active sites, we investigated the role of chromatin remodeling radiation-induced DNA damage. Nucleolin-depleted cells remarkably reduced cell cycle checkpoints and HR repair activity, and showed un-stabilization of stalled replication fork and abnormal chromatin remodeling. Therefore, nucleolin could be important to maintain genomic stability through chromatin remodeling.

Free Research Field

放射線生物学