A study on mechanisms of delayed micronucleus induction using live-cell imaging methods

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka Prefecture University
• Principal Investigator: YAGI Takashi 大阪府立大学, 理学(系)研究科(研究院), 教授 (80182301)
• Co-Investigator(Kenkyū-buntansha): KODAMA Seiji 大阪府立大学, 理学系研究科, 教授 (00195744) ; SUGIMOTO Kenji 大阪府立大学, 生命環境科学研究科, 教授 (40196746) ; KAWANISHI Masanobu 大阪府立大学, 理学系研究科, 准教授 (70332963)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 小核 / 遅延性小核誘導 / 環境変異原 / ライブセルイメージング

Outline of Final Research Achievements

Micronucleus induction is a marker of genotoxicity. We found that the micronuclei are induced at the first cell division as well as at the second and third divisions after treatment of environmental mutagens. This phenomenon is called delayed micronucleus induction. To elucidate this mechanism, mammalian cells were treated with the agents causing different types of cellular damage, e.g., X-ray, UV, colchicine, cis-platin, methyl nitrosourea. The micronucleus induction at the first cell division was increased with the amount of DNA damage; but that at the second cell division was increased with the amount of inhibition of spindle formation.

Free Research Field

分子細胞遺伝学