Generation of a new category, "Proteinaceous Insecticide" based on mechanisms which cause specificity diversity of Bacillus thuringiensis insecticidal protein.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310054
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: SATO Ryoichi 東京農工大学, (連合)農学研究科(研究院), 教授 (30235428)
• Co-Investigator(Kenkyū-buntansha): IWABUCHI Kikuo 東京農工大学, 大学院農学研究院, 教授 (00203399)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Bacillus thuringiensis / Cry toxin / ABCC2 / receptor

Outline of Final Research Achievements

To generate “Proteinaceous Insecticide” we attempted to make a method which can improve activity of the insecticidal protein made by BT. A group of insecticidal protein was indicated to act insect host using ABC transporter C2 as a receptor. It was also suggested that susceptibility of lepidopteran insects to Cry1A toxins largely depend on ABCC2s they have on the cells. To make a method by which we can evolve the binding affinity of insecticidal protein toward ABCC2 of target insects, we established large scale production system and purification system for ABCC2.We also made a platform for the screening system for the improved insecticidal proteins.

Free Research Field

昆虫病理学，昆虫生理学