2015 Fiscal Year Final Research Report
Application of Rhodococcus erythropolis PR4 for bioremediations
| Project/Area Number |
24310060
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OGIHARA Jun 日本大学, 生物資源科学部, 准教授 (50256830)
FURUKAWA Soichi 日本大学, 生物資源科学部, 准教授 (40339289)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | バイオレメデーション / 有機溶媒耐性 / GroEL2 / Rhodococcus |
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| Outline of Final Research Achievements |
We investigated the proteins involved in the translocation of R. erythropolis PR4. The results of our proteogenomic analysis suggested that GroEL2 was upregulated more in cells that translocated inside of the pristane phase than in those located at the aqueous-alkane interface attached to the n-dodecane surface. The expression of GroEL2 in PR4 (pK4-EL2-1) was 15.5-fold higher than that in PR4 (pK4). The growths of PR4 were enhanced by the introduction of pK4-EL2-1. These results suggested that the overexpression of groEL2 led to changes in cell localization and enhanced growth. We subsequently examined the effects of the introduction of groEL2 and subsequent overexpression of GroEL2 on the alkane tolerance of various Rhodococcus strains. The results showed that the introduction of groEL2 led to an increase in alkane tolerance in some rhodococci. As results, we constructed a highly alkane-tolerant strain capable of translocating to and surviving in the presence of n-octane.
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| Free Research Field |
環境微生物学
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