Development of new technology using ion beam for the investigation of pathogenesis and an early diagnosis of asbestosis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Quantum beam science
• Research Institution: Gunma University
• Principal Investigator: DOBASHI Kunio 群馬大学, 保健学研究科, 教授 (00241894)
• Co-Investigator(Kenkyū-buntansha): SATO Takahiro 独立行政法人日本原子力開発機構, 放射線高度利用施設部, 研究員 (10370404) ; ISHIZUKA Tamotsu 福井大学, 医学部, 教授 (50302477) ; SHIMIZU Yasuo 獨協医科大学, 医学部, 講師 (80396621)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 大気microPIXE / 石綿肺 / アスベスト / 肺線維症 / 特発性間質性肺炎 / 粉塵 / 元素分布 / イオンビーム

Outline of Final Research Achievements

In-air microparticle-induced X-ray emission (in-air micro-PIXE) analysis is employed to assess the spatial distribution and content of small amount of element in lung biopsy specimens. We analyzed the pathogenesis of lung fibrosis induced by inhaled asbestos or dust by microPIXE. The analysis of lung tissues from mice administrated silica particle and lung fibrosis patients showed significant increase of iron level on silica particle. And the expression of markers of oxidative stress and apoptosis were co-localized with silica particles. Lung tissues from 12 lung fibrosis patients were subjected to in-air micro-PIXE analysis. Two IIP patients showed a large quantity of Si and the co-localization of Si and Fe deposition with Fas expression.

Free Research Field

呼吸器内科