Development of long-term bystander imaging system using heavy-ion microbeam

2015 Fiscal Year Final Research Report

• Project/Area Number: 24310073
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Quantum beam science
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: Funayama Tomoo 国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門・量子ビーム応用研究センター, 研究主幹 (40354956)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 量子ビーム医療応用 / 重イオンビーム / マイクロビーム / バイスタンダー効果 / 低線量放射線影響

Outline of Final Research Achievements

To explore the molecular mechanisms of heavy-ion induced bystander effect, a system for long-term live imaging of microbeam-irradiated cell sample was developed. As a first step, I developed a software framework for developing multiple software for different experimental purpose. Using the framework, a novel cell targeting software was build, which enables integrated sample irradiation with focusing heavy-ion microbeam. This software enabled a rapid and accurate irradiation of targeted cells with focusing heavy-ion microbeam. Thereafter, I developed software libraries for long-term real-time autofocusing and automatic imaging of the target sample. Finally, these codes were integrated into the software framework to establish a long-term bystander imaging system.

Free Research Field

放射線生物学