Construction and application of single molecule observation system based on DNA nanotechnology

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310097
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: ENDO Masayuki 京都大学, 物質-細胞統合システム拠点, 准教授 (70335389)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: DNAナノテクノロジー / ＤＮＡオリガミ / 1分子観察 / 高速原子間力顕微鏡 / ナノ構造体 / ナノ空間 / ＤＮＡ構造変化 / 酵素反応

Outline of Final Research Achievements

In this study, we intend to elucidate dynamic biological reactions in detail using DNA nanostructures, and visualize the behavior of single biomolecule on a DNA nanostructure using high-speed atomic force microscopy (AFM). We also operate of the interaction of biomolecules and chemical reactions at the single-molecule level. We created the DNA naospace for reaction and movement of target molecules, and developed a method to directly observe the behavior of DNA structural change such as the B-Z transition and the enzyme reaction such as transcription and DNA recombination. Furthermore, we successfully created two-dimensional and three-dimensional DNA nanostructures that have photoresponsive functions, and created a system to visualize their behaviors using high-speed AFM.

Free Research Field

DNAナノテクノロジー