The effects of the intracytoplasmic sperm injection on the gene expression regulation in the human preimplantation embryo.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310141
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KOHDA Takashi 東京医科歯科大学, 難治疾患研究所, 准教授 (60211893)
• Co-Investigator(Renkei-kenkyūsha): KUBOTA Toshiro 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (50126223) ; TSUTSUMI Osamu 国際医療福祉大学, 大学院医療福祉学研究科, 教授 (60134574)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 顕微受精 / 体外受精 / 遺伝子発現

Outline of Final Research Achievements

The application of the in vitro fertilization (IVF) in assisted reproductive technology has been increased since its first introduction. Recently, the intracytoplasmic sperm injection (ICSI) technique is used in about half of the IVF cases in Japan.
The aim of this study is to evaluate the effects of ICSI on the transcription regulation in human early development and to improve the ICSI method. First, we successfully established the RNA amplification protocol for single embryo RNA-seq analysis using T7 RNA polymerase. Then we analyzed the gene expression profile of the human blastocysts that were not transferred in assisted reproductive technologies. As the results, we identified the genes that affected their expression by ICSI procedure. These genes expected to be the useful markers to improve the ICSI technique.

Free Research Field

エピジェネティクス