New site-specific recombination systems for genome engineering, VCre/VloxP and SCre/SloxP

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310143
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Kazusa DNA Research Institute
• Principal Investigator: NAKAYAMA MANABU 公益財団法人かずさDNA研究所, 技術開発研究部, チーム長 (30370927)
• Co-Investigator(Renkei-kenkyūsha): KOSEKI Haruhiko 理化学研究所・統合生命医科学研究センター, 免疫器官形成研究グループ, グループディレクター (40225446)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオテクノロジー / 部位特異的組み換え / 病態モデル動物 / 有用遺伝子探索 / 染色体工学 / 遺伝子改変動物 / ゲノム編集 / ゲノム操作技術

Outline of Final Research Achievements

Two popular tools used in genomic engineering are the Cre/loxP and Flp/FRT site-specific recombination systems, which are often used to develop conditional knockout mice. Alternative combinations of recombination systems, in addition to the Cre/loxP and Flp/FRT systems, can be useful tools for genetically modifying genomes. We have developed two novel site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. VCre and SCre recombinases originate from Vibrio sp. and Shewanella sp. ANA-3, respectively. Because their recognition sites are different from Cre recognition sites, we can use these site-specific recombination systems simultaneously. Moreover, we have identified some additional site-specific recombination systems derived from other species. These new site-specific recombination systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells.

Free Research Field

機能ゲノム学