2014 Fiscal Year Final Research Report
Secondary structure-based analysis of small RNA from mouse brain
| Project/Area Number |
24310147
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
System genome science
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| Research Institution | Chiba Institute of Technology |
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Principal Investigator |
KAWAI Gota 千葉工業大学, 工学部, 教授 (70211860)
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| Co-Investigator(Kenkyū-buntansha) |
KIYOSAWA Hidenori 高知大学, 医学部, 特任准教授 (30295422)
USHIDA Chisato 弘前大学, 農学生命科学部, 准教授 (50250593)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 機能性RNA / NMR / 立体構造解析 / トランスクリプトーム |
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| Outline of Final Research Achievements |
Next-generation sequencing was applied to small RNA fractions with lengths ranging from 40 to 140 nt from mouse brain and secondary structure-based clustering was performed. From the hundred million sequences obtained, 16 candidates of the mouse structured small non-coding RNAs (MsncRs) were isolated. Their expressions, together with those of other candidates, were analyzed by northern hybridization. One among 16 candidates obtained by the next-generation sequencing revealed a signal shorter than expected.
One of the candidates, MsncR-11, have a sequence complementary to a mitochondrial tRNA. NMR analysis suggested that MsncR-11 forms a secondary structure similar to tRNA including acceptor and T stems.
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| Free Research Field |
構造生物学
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