Genome wide analysis of translation efficiency by local structure of mRNA and nascent peptide

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310148
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: System genome science
• Research Institution: Keio University
• Principal Investigator: NAKAHIGASHI Kenji 慶應義塾大学, 政策・メディア研究科, 特任准教授 (70322740)
• Co-Investigator(Kenkyū-buntansha): MORI Hirotada 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (90182203) ; KANAI Akio 慶應義塾大学, 環境情報学部, 教授 (60260329)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: リボソームプロファイリング / ゲノム解析技術 / リボソーム / 翻訳 / 翻訳制御 / コドン

Outline of Final Research Achievements

We performed genome wide analysis of efficiency in mRNA translation to protein, employing new method. We experimentally showed that rate of initiation largely affected the overall efficiency of a gene, while difference in elongation efficiency by codon usage did exist but the effect was much smaller.
We also found new insights in the nature of translation apparatus and factors, such as efficiency of non-canonical codon-anticodon recognition at 3rd letter and translational regulation by sRNA/Hfq, as well as effect of nascent-peptide sequence on specificity of various antibiotics.

Free Research Field

システム生物学