Search for bioactive natural products acting at infected region of M. tuberculosis and analysis of their target molecules

2015 Fiscal Year Final Research Report

• Project/Area Number: 24310159
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: Arai Masayoshi 大阪大学, 薬学研究科(研究院), 准教授 (80311231)
• Co-Investigator(Renkei-kenkyūsha): KOBAYASHI Motomasa 大阪大学, 大学院薬学研究科, 教授 (40116033)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 抗菌物質 / 結核 / 感染症 / 活性天然物 / ケミカルバイオロジー

Outline of Final Research Achievements

In order to search for new anti-microbial substances against the dormant Mycobacterium tuberculosis, which shows tolerance to the conventional anti-Tuberculosis drugs, we constructed several screening systems focusing on the characteristic phenotypes of mycobacteria at the infected region. On the guidance of the screening from the extract library of marine sponges and marine-derived microbes, we could explore several anti-microbial compounds including structurally new compounds, which are expected to be a new medicinal seed. In addition, we could clarify the target molecules of some isolated anti-microbial compounds by the method utilizing the transformants overexpressing genomic DNA library, the analysis of gene mutation and/or the pull-down assay using a synthetic probe of the active compound. The clarified target proteins are expected to become a new drug target against the dormant M. tuberculosis.

Free Research Field

生物有機化学