New Principle of Microorganism Identification: Chemistry based on Small Fluorescent Molecule and Siderophore

2014 Fiscal Year Final Research Report

• Project/Area Number: 24310162
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Chemical biology
• Research Institution: The University of Tokushima (2013-2014); Hokkaido University (2012)
• Principal Investigator: NAMBA Kosuke 徳島大学, ヘルスバイオサイエンス研究部, 教授 (50414123)
• Co-Investigator(Kenkyū-buntansha): MURATA Yoshiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主席研究員 (60256047) ; ODA Masataka 新潟大学, 大学院医歯薬系, 准教授 (00412403)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: シデロフォア / 1,3a,6a-トリアザペンタレン / 蛍光プローブ / ビブリオフェリン / エンテロバクチン

Outline of Final Research Achievements

Novel smallest fluorescent molecules were developed, and they were introduced to siderophore that is secreted by microorganism as a natural iron chelator. We succeeded to produce various compact fluorescent molecules that cover all color region from blue to red on the basis of new fluorescent chromophore 1,3a,6a-triazapentalene originally developed in our laboratory . In addition, vibrioferrin and enterobactin as the siderophore of Vibrio parahaemolyticus and salmonella respectively were efficiently synthesized. Furthermore, the introduction of various TAPs to vivrioferrin and enterobactin was also achieved. The detection and identification of specific microorganism by using TAP-siderophore are currently underway in our laboratory.

Free Research Field

ケミカルバイオロジー