2014 Fiscal Year Annual Research Report
磁気感受性を担う細胞内タンパクの分光学的同定と空間特性
| Project/Area Number |
24350002
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
ウッドワード ジョナサン 東京大学, 総合文化研究科, 准教授 (80526054)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | TOAD Microscopy / MIM microscopy / Imaging / Magnetic Field Effect / Low Field Effect / Radical Pair Dynamics / Drosophila Cryptochrome / Flavin photochemistry |
|---|
| Outline of Annual Research Achievements |
Optimisation of the microscope was completed. Past experimental difficulties were successfully overcome. A new double modulation method (Magnetic Intensity Modulation (MIM) microscopy) was developed which exploits fast switching of the pump laser. This approach also enabled the study of microsecond timescale reaction kinetics at any arbitrary sample location. Studies were undertaken on FAD photochemistry at low pH where the microscope was able to make very sensitive measurements at high speed. For the first time a low field effect (LFE) was resolved in this important photoreaction. A reverse sense magnetic field effect (MFE) on FAD fluorescence was observed. Given the significance of the results,the first manuscript was prepared for and submitted to a high profile chemistry journal instead of a technical journal. Kinetic simulations of the observed behaviour were performed and included in this manuscript.
The high sensitivity of the optical subsystem was exploited to allow small changes in Bhalf values to be observed in a cuvette-based experiment. This approach was used to provide new insights into the reaction of FAD with tryptophan (new collaboration with Prof. Kiminori Maeda, Saitama University)
Studies commenced on of drosophila derived cryptochrome (DmCry) protein samples in vitro, provided by Manchester collaborators. A MFE and a LFE on DmCry photochemistry was observed with surprising laser power dependence. Due to the significance of this breakthrough, these measurements have been prioritised over arabadopsis thaliana cell culture measurements in the short term.
|
| Research Progress Status |
26年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
26年度が最終年度であるため、記入しない。
|
| Causes of Carryover |
26年度が最終年度であるため、記入しない。
|
| Expenditure Plan for Carryover Budget |
26年度が最終年度であるため、記入しない。
|
Research Products
(4 results)
