2014 Fiscal Year Final Research Report
Biological molecular layers in aqueous environments studied by high-sensitive vibrational SFG spectroscopy
| Project/Area Number |
24350010
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 非線形分光 / 振動スペクトル / 界面 / 生体分子 / 単分子膜 |
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| Outline of Final Research Achievements |
Several techniques for high-sensitive vibrational sum-frequency generation (SFG) spectroscopy to investigate biological molecular layers in aqueous conditions have been developed or examined. The techniques include (1) high-sensitive heterodyne-detected vibrational SFG spectrometer with a very-thin quartz crystal as the local oscillator, (2) nano-particle enhanced vibrational SFG, and (3) prism-parallel substrate coupled systems for vibrational SFG at total reflection geometries. As applications, surface-pressure dependent structures of lipid layers on water and silica substrates (Langmuir and Langmuir-Blodgett monolayers) and molecular orientations and structures of proteins and water at surface of proteins aqueous solutions have been investigated.
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| Free Research Field |
物理化学
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