2014 Fiscal Year Final Research Report
Development of theree-dimentional cell culture chip for drug screening
| Project/Area Number |
24350037
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Shuji 東京大学, 大学院総合文化研究科, 准教授 (90447318)
TOMITA Shunsuke 独立行政法人産業総合技術総合研究所, バイオメディカル研究部門, 研究員 (50726817)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 幹細胞 / マイクロパタン / 三次元培養 / 分泌液 / secretome / 合成高分子 / 酵素 / 分化 |
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| Outline of Final Research Achievements |
The effects of three-dimensional environment in culture for ADSCs were researched on microfabricated surface. We successfully accomplished the 3-D construction of ADSCs (ADSC spheroid) with high viability and differentiate the ADSC spheroid into osteoblasts efficiently. The Live/Dead staining assay revealed that cells in the ADSC spheroid maintained high viability for at least 28 days in culture. After the additional cultivation of ADSC spheroid in osteogenic differentiation medium for 14 days, the ADSC spheroids were characterized the extent of differentiation by specific staining for osteoblasts and RT-PCR. We have also applied a PIC sensor array to recognize “secretomic signatures” of culture supernatants for markerless and noninvasive identification of differentiated MSCs using only a standard microplate reader. Theseis technology and the findings in this study could provide an interesting new approach to the construction of bone tissues for regenerative medicine.
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| Free Research Field |
バイオ分析化学
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