Development of high performance bioanalytical techniques by capillary/microchip separation and detection systems

2014 Fiscal Year Final Research Report

• Project/Area Number: 24350039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyoto University
• Principal Investigator: OTSUKA Koji 京都大学, 工学(系)研究科(研究院), 教授 (70183762)
• Co-Investigator(Kenkyū-buntansha): KUBO Takuya 京都大学, 工学研究科, 准教授 (20374994) ; NAITO Toyohiro 京都大学, 工学研究科, 助教 (10711806)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 分離科学 / キャピラリー / マイクロチップ / 電気泳動 / クロマトグラフィー / バイオ分析

Outline of Final Research Achievements

Development of high performance bio-separation and analysis systems based on capillary electrophoresis (CE) and microchip electrophoresis (MCE) was investigated, where developments of high performance separation and detection systems using online sample concentration techniques and mass spectrometry (MS) and novel separation fields for separations of proteins and carbohydrates were mainly carried out. Following four categories were most important issues: 1) progress of high performance online sample concentration techniques; 2) improvement of the performance of MCE-MS using an interface integrated microchip with a separation channel for MCE; 3) development of CE/MCE-MS using online sample concentration techniques; 4) improving the performance of CE/MCE with novel selective separation fields.

Free Research Field

分析化学