The shortest synthetic route of polymeric glycomaterials through a one-pot reaction system based on organic reaction and enzymatic reaction

2015 Fiscal Year Final Research Report

• Project/Area Number: 24350052
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Polymer chemistry
• Research Institution: Tohoku University
• Principal Investigator: Shoda Shin-ichiro 東北大学, 工学(系)研究科(研究院), 教授 (10143364)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 化学酵素合成 / 糖タンパク質 / ヒトミルクオリゴ糖 / 糖オキサゾリン / 脱水縮合剤 / 熱応答性高分子 / 糖転移反応 / 糖加水分解酵素

Outline of Final Research Achievements

A basic research on the development of one-pot glycosylation based on chemical reaction and enzymatic reaction has been carried out. An oligosaccharide having an N-acetylglucosamine moiety at the reducing end was converted to the corresponding sugar oxazoline derivative in more than 95% yields by using 2-chloroimidazolinium salt or 2-chlorobenzimidazolium salt as dehydrating agents. As a model compound for controlling enzyme activities, several thermosensitive glycopolymers based on poly(N-isopropylacrylamide) having a chitooligosaccharide was prepared. Based on these results,glycoproteins having a definite oligosaccharide as well as human milk oligosaccharides have successfully been prepared in good yields.

Free Research Field

糖鎖工学　高分子合成