An enzymatic method for preparation of 6-membered carbocyclic compound from D-glucose

2014 Fiscal Year Final Research Report

• Project/Area Number: 24350078
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: EGUCHI Tadashi 東京工業大学, 理工学研究科, 教授 (60201365)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 糖質環化酵素 / グリーンケミストリ / ポリリン酸グルコキナーゼ

Outline of Final Research Achievements

A potential one-step process to 6-membered carbocyclic compound, 2-deoxy-scyllo-inosose, from D-glucose comprising one-pot incubation of D-glucose with recombinant 2-deoxy-scyllo-inosose synthase (BtrC) and polyphosphate glucokinase in the presence of polyphosphate and NAD+ was developed. It should be emphasized here that the present approach has significant potential for the production of 6-membered carbocyclic compound from widely available D-glucose and further implies significant opportunity for developing acceptable ‘green’ ways for the production of organochemical resources from sustainable agricultural and biomass products by utilizing enzymes involved in natural product biosynthesis.

Free Research Field

生物有機化学