Analysis of RNA editing and chemical modification at the single molecule level

2015 Fiscal Year Final Research Report

• Project/Area Number: 24350084
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Osaka University
• Principal Investigator: Kawai Kiyohiko 大阪大学, 産業科学研究所, 准教授 (50314422)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 遺伝子診断 / RNA / エピジェネティクス / １分子計測(SMD) / DNA

Outline of Final Research Achievements

A new detection method was developed for detection of RNA at the single-molecule level. We foucused on that the charge transfer rate through DNA is strongly affected by the DNA sequence and chemical modification of nucleobases. The charge-transfer dynamics in DNA/RNA hybrid was measured as the length of the duration of the off time of blinking of the fluorescence. This enabled the detection of target sequence, allowing for sample sizes as small as 5 fmol (< 0.2 nM, 20 uL). The target can be analyzed under conditions of the automatic measurement of more than 100 samples within 3 h, which makes possible the high-throughput screening needed in RNA diagnosis.

Free Research Field

生物有機化学、光化学、核酸化学